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Dot blot is a quick method for detecting biological samples like proteins or nucleic acids. It follows a similar principle to Western blotting, except the sample is not blotted from a gel. Instead, samples are dotted directly onto a membrane before being probed for detection. In applications involving several steps - from producing and labeling a probe to detecting the labeled probe - assessing labeling efficiency can be an essential part of assay design. The efficiency of labeling can be estimated by comparing the relative detection sensitivity of the labeled nucleic acid to a standardized sample in a side by side dot blot dilution series.
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Casino (1995) Movie || Robert De Niro, Sharon Stone, Joe Pesci || Review and Facts Casino is a 1995 epic crime drama film directed by Martin Scorsese, adapted by Scorsese and Nicholas Pileggi from the latter's nonfiction book Casino: Love and Honor in Las Vegas.[6] It stars Robert De Niro, Sharon Stone, Joe Pesci, Don Rickles, Kevin Pollak, Frank Vincent and James Woods. The film was the eighth collaboration between director Scorsese and De Niro. Casino follows Sam "Ace" Rothstein (De Niro), a Jewish American gambling expert handicapper who is asked by the Chicago Outfit to oversee the day-to-day casino and hotel operations at the Tangiers Casino in Las Vegas. Other significant characters include Nicky Santoro (Pesci), a "made man" and friend of Sam, and Ginger McKenna (Stone), a streetwise chip hustler whom Sam marries and has a daughter with. The film details Sam's operation of the casino, the difficulties he confronts in his job, the Mafia's involvement with the casino, and the gradual breakdown of his relationships and standing, as Las Vegas changes over the years. Movie Credits! Directed by: Martin Scorsese Produced by: Barbara De Fina Starring: Robert De Niro, Sharon Stone, Joe Pesci, Don Rickles, Kevin Pollak, James Woods, Frank Vincent Production companies: Universal Pictures, Syalis D.A., Légende Entreprises, De Fina/Cappa Distributed by: Universal Pictures (International), Ariane Films (France) Release dates: November 14, 1995 (New York premiere), November 22, 1995 (United States), March 13, 1996 (France)